Coding

Part:BBa_K5382136

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-26)


Cas9 handle-reverse repeating sequence in gRNA

The Cas9 handle, specifically the stem-loop sequence of the guide RNA (gRNA), refers to the palindromic sequence within its structure. This sequence is capable of folding back upon itself to form a secondary structure, namely a stem-loop configuration, due to the hydrogen bonding between complementary nucleotide bases. Within the gRNA, this conserved sequence facilitates the stabilization of the gRNA-Cas9 protein complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None